WebMay 4, 2013 · 2013-05-04. 【质粒共享】pGEX-6P-1载体编号:P0048 载体名称:pGEX-6P-1 载体抗性:Amp 载体大小:4900bp 载体宿主:E.coli 载体特点:A tac promoter for chemically inducible, high-level expression. An internal lac Iq gene for use in any E. coli host. Very mild elution conditions for release of fusion proteins from ... http://journals.im.ac.cn/html/wswxtbcn/2024/1/tb20010013.htm
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WebSwaI is typically used at 25°C, but is 50% active at 37°C. After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. SmaI can be used at 37°C for brief incubations. WebSwaI is typically used at 25°C, but is 50% active at 37°C. After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. … mobilesoftwareupdate as the mountpoint
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WebAug 4, 2024 · 1、复制起点(Origin of replication,ori). 负责控制质粒复制的起始,使质粒能够复制,并决定了质粒的宿主和拷贝数。ori序列富含腺嘌呤(A)和胸腺嘧啶(T),由 … http://www.vectorhub.cn/plasmid-profile-search.html WebSep 20, 2016 · Double digest the pYLEX1 plasmid with PmlI and KpnI enzymes as per Table 4. Purify the digestion mixture using a PCR purification kit as per manufacturer's … ink copywriters ltd