Phis2.1 vector

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August undefined, 2024

Webb13 juni 2015 · GTP1-GTP8 indicates the pGAD-OsGRAS23 plus pHIS2.1-cis (promoters of Os07g0162450, Os01g0537250, Os07g0638400 ... the promoter sequences were cloned into the yeast expression vector pHIS2.1 between the SmaI and EcoRI sites. The constructs were co-transformed into the yeast Y187 (MATa; ura3–52; his3–200; … Webb19 sep. 2024 · The pHIS2 vector carries the TRP1 nutritional marker for selection in yeast. The transformed yeast cells were cultured on SD/-Trp-Leu medium and SD/-Trp-His-Leu medium supplemented with 10 mM or 30 mM 3-amino …

pHIS2 vector map and sequence - novoprolabs.com

Webb16 mars 2024 · Y187-pHis2系统酵母单杂培养基套装说明书. 储存温度：序号1-7常温保存；序号8需-20℃储存，蓝冰运输. 产品组成：（0.5 L/pouch）产品说明： Y187-pHis2 … WebbpHIS2 vector (V001297#) Basic Vector Information. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The … early nasa space mission

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WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community … http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf WebbWe'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2024 ... cst simplechip

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Y187-pHis2系统酵母单杂培养基套装说明书-分析方法-生物在线 …

WebbUnique restriction sites are in bold. pHIS2.1 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was … Webb1.构建载体目的基因融合连入pGADT7-Rec2(AD); 顺式作用元件连入pHis2或pHis2.1载体(顺式元件可以只连接一个拷贝，也可以连接3个串联的并测序确认无误)。 2.验证两载体分 … early names of nasa space prograesWebbVectors in Use. N or C-terminal His tag. Parallel vectors 1 & 3 contain N-terminal His (pET22), MBP (pMal) and GST (pGex) with similar MCS for easy cloning. Parallel vector 2 contains N and C-terminal His (pET22), … early name for bicycles

"Webb25 okt. 2016 · In the mutated assay, the sequence of the G-box in the NWRE (GCGTTCTTACTTTTCGTAAGGG CACGTG AAGCACCCCAACAAA) was replaced with … " - Phis2.1 vector

Phis2.1 vector

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Webb24 jan. 2024 · For ligation: the molar ratio of your bait sequence: vector ranges from 3:1 and 5:1. The reaction can be put at 16 degrees overnight (in PCR cycler machine). It … Webb16 feb. 2024 · Two GA-inducible genes expansin 1 (PhEXP1) and cysteine proteinase 1 (PhCP1) were down-regulated in the flowers infected with TRV-PhCHS/GA20ox3 . …

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Webb1.一袋培养基干粉倒入三角瓶或培养瓶中，加0.5L去离子水中溶解（琼脂冷水不溶）。 2.无调整pH值，121℃高压灭菌15min。 3.液体培养基封好口避光、室温储存备用。 4.固体 … Webb一、载体概念. 1. 载体，DNA的搬运工. 载体：在基因工程重组DNA技术中，将DNA片段（目的基因）转移至受体细胞的一种能自我复制的DNA分子。. 2. 载体，基因密码承载者. 载 …

WebbChina Agricultural University (CAU, Chinese: 中国农业大学; pinyin: Zhōngguó Nóngyè Dàxué; abbreviated 农大/Nóngdà) is a university in Beijing, People's Republic of China … WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the …

WebbIn this construct, three copies of the DNA target (T) have been inserted into the pHIS2.1 reporter vector. GAL UAS minimal promoterADE2, HIS3, lacZ, and MEL1 transcription … Webb27 apr. 2024 · As with the positive control, yeast cells co-transformed with AD-PpPIF8 and proPpCHS-pHIS2.1 vectors could also grow on both selective mediums. However, the negative control yeast cells co-transformed with empty AD and proPpCHS -pHIS2.1 vectors could only grow on SD/−Leu−Trp medium ( Figure 11 F).

Webb质粒简介 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with …

http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html early native american history factsWebb21 mars 2024 · 其中，香豆素（1,2-苯并吡喃酮）的生理浓度足以抑制苹果枝在hk组织中的生长，外源施用可以提高lk苹果枝条对苹果枝的抗性。过表达β-葡萄糖苷酶40（MdBGLU40）的转基因苹果愈伤组织编码香豆素合成酶，含有更高水平的香豆素，即使在LK条件下也表现出对苹果枯萎病的高抗性。 early names for australiaWebb8 juli 2024 · pHis2 编号载体名称北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体高拷贝/低拷贝: -- 启动子: -- … early nasa rocket testshttp://www.biofeng.com/zaiti/jiaomu/pYES2.html cst sim pedalsWebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the … early national television news anchorsWebb8 juli 2024 · pHis2 编号载体名称北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点，限制性内切酶载体大小: 7207bp 5' 测序引物及序列: -- 3' 测序引物及序列: -- 载体标签: -- 载体抗性: 卡纳霉素筛选标记: -- 备注: 含HIS3 报告基因产品目录号: -- … cstskins.comWebbClose. We will increase some of our prices on April 1, 2024. Be sure to complete your order before this time to take advantage of current prices. See the new prices and get more … csts isic