Human normal hepatocytes l02
WebOne normal hepatocyte cell line (L02) and three HCC cell lines (HepG2, HuH7, and Hep1) were obtained from the Institute of Cell Biology of the Chinese Academy of Sciences. Cells were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 g/mL streptomycin (Invitrogen, Carlsbad, CA, … Web方法. 采用0.1 mmol/L H 2 O 2 干预1 h构建人肝细胞L02氧化损伤模型,并采用不同浓度的解毒化瘀工作液(Jie-Du-Hua-Yu, JDHY)进行处理。 将细胞分为5组:正常组、H 2 O 2 组、H 2 O 2 + JDHY(0.5 mg/ml)组、H 2 O 2 + JDHY (1 mg/ml)组、H 2 O 2 + JDHY (1.5 mg/ml)组。 MTT检测细胞活性,透射电镜观察细胞线粒体形态,生物化学 ...
Human normal hepatocytes l02
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WebHuman Normal Hepatocyte Derived Cell Line L02, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … Web2 jul. 2024 · Primary human hepatocytes are the gold standard for drug development studies. Olson et al. compared drug toxicities between humans and various animals, including dogs, primates, rats, mice, and guinea pigs [ 46 ]. Their analysis indicated that the overall concordance between human and animal toxicity was 71%.
Web26 okt. 2024 · Sunitinib significantly induced L02 cells death by triggering apoptosis and autophagy acted as a self-defense mechanism to promote survival. Sunitinib exposure … Web25 jan. 2024 · cytotoxicity: Protecting human normal L02 hepatocytes . versus sensitizing human hepatoma HepG2 cells. YUEYING LI 1, XINCHI ZHANG 2, XIAOMING Y ANG 3, JUN LIU 4, LINJIE LI 5, WENBIN MA 5 and MIN ...
WebIt is reported that benzene can cause hematopoietic disorders and has been recognized as a human carcinogen. However, the mechanisms by which it increases the risk of carcino … Web26 okt. 2024 · The L02 cells (purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd.) are human normal hepatocytes widely used for studying hepatic …
Web27 aug. 2024 · Differentially expressed hsa_circRNA_002178, in 7 HCC tissue samples and paracancerous tissues, as well as in HCC cell lines and normal hepatocytes, was checked by RT-qPCR. Cell proliferation, invasion, migration, and epithelial-to-mesenchymal transition (EMT)-related proteins were tested in hsa_circRNA_002178-overexpressed or …
Web29 jul. 2024 · Most SGLT-2i inhibited cell proliferation by activating caspase 3 in human hepatocellular carcinoma cells—HepG2 cells . Li, L. et al. demonstrated that dapagliflozin, an SGLT-2i, reduced lipid droplet content in HepG2 and L02 (an immortalized normal human hepatocyte-derived hepatocyte) cells. perlymae44 sbcglobal.netWebhuman normal hepatocyte line l02 ( ATCC ) ATCC is a verified supplier ATCC manufactures this product About News Press Release Team Advisors Partners Contact … perlypso love storyWeb20 apr. 2024 · Alteration of liver tissue mechanical microenvironment is proven to be a key factor for causing hepatocyte injury and even triggering the occurrence of hepatocellular carcinoma; however, the underlying mechanisms involved are not fully understood. In this study, using a customized, pressure-loading device, we assess the effect of pressure … perlynWebHuman hepatocytes L02 cells obtained from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) were cultured in HyClone RPMI 1640 medium (GE Healthcare, Beijing, China) supplemented with 10% FBS (Biological Industries, Cromwell, CT, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a … perlycoWebNAFLD model was induced by PA and was added to the media at a final concentration of 400 μM in hepatic L02 cell lines. 20 Hepatic L02 cells (3×10 3 /well) were plated into 96-well plates and were divided into five groups: normal group (untreated cells, fresh medium only), PA group and PA plus Sal intervention groups (low dose group, middle dose group and … perlys reactilWeb19 aug. 2009 · Human embryonic hepatocytes (L02 cells) were cultured in RPMI 1640 medium supplemented with 10% fetal calf serum, 100 U/ml penicillin, and 100 μg/ml streptomycin. Cultures were maintained at 37°C in 5% CO 2 incubator. Analysis of Cell Viability Viability of cells was evaluated by MTT reduction assay. perlys design reactilWeb11 jul. 2024 · Normal human liver cell line L02 and human HCC cell lines SMMC-7721 and Huh-7 were cultured in high-glucose Dulbecco’s modified Eagle’s medium containing 10% FBS, 100 units/mL penicillin,... perlys chur